计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
皮质醇(Cortisol)是一种类固醇激素,属于糖皮质激素类。当作为一种药物使用时,它被称为氢化可的松。它在许多动物体内产生,主要由肾上腺皮质的筋膜区产生。尿游离皮质醇由血液中游离皮质醇经肾小球滤过而来,因此基量与血浆中真正具有生物活性的游离皮质醇成正比。皮质醇在调节葡萄糖代谢中起着至关重要的作用,并促进肝脏中的糖异生(葡萄糖合成)和糖原生成(糖原合成)以及骨骼肌中的糖原分解(糖原分解)。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有大鼠尿游离皮质醇(UFC)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠尿游离皮质醇(UFC)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat UFC antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat UFC in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.