BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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抗利尿激素（ADH），又称血管加压素、精氨酸血管加压素（AVP）或精氨酸加压素，是一种由AVP基因在下丘脑的神经元中合成的肽原激素，并转化为AVP。然后它沿着该细胞的轴突下行，终止于垂体后叶，并在细胞外液高渗（高溶血性）的情况下从囊泡中释放到循环中。AVP有两个主要功能。首先，它增加了从肾小管的滤液中重吸收回循环的无溶质水的数量。第二，AVP使动脉血管收缩，从而增加外周血管阻力，提高动脉血压。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有大鼠抗利尿激素;血管加压素;精氨酸加压素(ADH;VP;AVP)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠抗利尿激素;血管加压素;精氨酸加压素(ADH;VP;AVP)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat ADH;VP;AVP antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat ADH;VP;AVP in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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