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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
晚期糖基化终末产物(AGEs)是由于暴露于糖而变得糖化的蛋白质或脂质。AGEs几乎影响到体内每一种类型的细胞和分子,并被认为是衰老和一些与年龄有关的慢性疾病的一个因素。它们还被认为在糖尿病的血管并发症中起着致病作用。AGEs在某些病理条件下产生,如糖尿病患者高血糖导致的氧化应激,它在妊娠期糖尿病中也发挥着促炎症介质的作用。在心血管疾病方面,AGEs可诱导胶原蛋白交联,从而导致血管僵化和低密度脂蛋白颗粒(LDL)在动脉壁上的夹带。AGEs还可以引起低密度脂蛋白的糖化,从而促进其氧化。氧化的低密度脂蛋白是动脉粥样硬化发展的主要因素之一。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有大鼠晚期糖基化终末产物(AGEs)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠晚期糖基化终末产物(AGEs)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat AGEs antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat AGEs in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.