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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
生长抑素(SST),又称生长激素抑制激素 (GHIH),是一种调节内分泌系统的肽类激素。它通过与G蛋白偶联生长抑素受体相互作用和抑制许多次级激素的释放来影响神经传递和细胞增殖。它抑制胰岛素和胰高血糖素的分泌。它有两种活性形式,由单个前蛋白的替代切割产生:一种由14个氨基酸组成,另一种由28个氨基酸组成。它的释放受到迷走神经的抑制。它的作用扩散到身体的不同部位。在消化系统中,它由几个位置的δ细胞分泌,即幽门窦,十二指肠和胰岛,它与皮质抑素同源并抑制胃肠道激素的释放、降低胃排空率,减少平滑肌收缩和肠内血流量、抑制胰腺激素的释放、抑制胰腺的外分泌分泌作用。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有大鼠生长抑素(SS)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠生长抑素(SS)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat SS antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat SS in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.