计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
神经肽Y(NPY)是一种36个氨基酸的神经肽,参与中枢和周围神经系统的各种生理和平衡过程。它已被确定为存在于哺乳动物中枢神经系统中最丰富的肽,该系统包括大脑和脊髓。它与其他神经递质如GABA和谷氨酸一起分泌。在自律神经系统中,它主要由交感神经系统的神经元产生,作为一种强烈的血管收缩剂,也导致脂肪组织的生长。在大脑中,它在包括下丘脑在内的不同位置产生,被认为有几种功能,包括:增加食物摄入和以脂肪形式储存能量,减少焦虑和压力,减少痛觉,影响昼夜节律,降低血压,以及控制癫痫发作。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有大鼠神经肽Y(NPY)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠神经肽Y(NPY)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat NPY antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat NPY in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.