BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肾上腺素(adrenaline)，L-3,4-二羟基-alpha-苄醇，是肾上腺髓质分泌的激素及神经传导物质，也是一种药物。肾上腺素被应用于治疗多项疾病，包含全身性过敏反应、心搏停止，以及表面出血等等，吸入式的肾上腺素有时会被用于改善义膜性喉炎的症状。另外当哮喘的第一线治疗皆无效时，也可能会考虑使用肾上腺素。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有大鼠肾上腺素(EPI)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠肾上腺素(EPI)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat EPI antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat EPI in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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