BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肾上腺髓质素(ADM)是一种血管扩张肽激素，由52个氨基酸组成的肾上腺髓质素是在1993年被首次发现的。它有降低肺部血压，减轻炎症并减少液体渗漏进肺等作用。这种天然肽还通过改善慢性阻塞性肺病，高血压和心肌梗死等疾病，在保护和调节心肌和肺功能方面扮演重要角色。肾上腺髓质素还被认为能抑制癌细胞的增殖。它通过降血钙素受体和受体活性修饰蛋白相互作用被建议介导作用。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有大鼠肾上腺髓质素(ADM)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠肾上腺髓质素(ADM)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat ADM antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat ADM in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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