BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胃动素(MTL)是一种22个氨基酸的多肽激素，它由内分泌Mo细胞分泌，这些细胞在小肠隐窝，特别是在十二指肠和空肠中很多，其作用是促进和影响胃肠运动及胃肠道对水、电解质的运输。这种生理作用主要通过激发消化间期肌电活动Ⅲ相，促进胃强力收缩和小肠分节运动，该运动可周期性产生并向小肠远端传播，从而可加速小肠的传递时间，另外胃动素尚有增加结肠运动的作用，故血浆胃动素水平升高，肠道蠕动加速，使肠内容物通过加快，临床表现为腹痛、腹泻等症状。结果发现,肠易激综合征腹泻型血浆胃动素水平升高，显著高于便秘组及正常人组，证实胃动素升高是肠易激综合征腹泻型的发病原因之一。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有大鼠胃动素(MTL)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠胃动素(MTL)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat MTL antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat MTL in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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