计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胃泌素是一种多肽激素,可刺激胃顶细胞分泌胃酸(HCl)并帮助胃部运动。它是由十二指肠和胃幽门前的G细胞产生的一种线性肽类激素。它被分泌到血液中,编码的多肽是前胃泌素,在翻译后修饰中被酶裂解,产生前胃泌素(一种中间的、无活性的前体),然后产生各种形式的胃泌素,主要有三种:胃泌素-34、胃泌素-17、胃泌素-14。另外,五肽胃泌素是一种人工合成的、与胃泌素C端最后五个氨基酸序列相同的氨基酸序列。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有大鼠胃泌素(Gas)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠胃泌素(Gas)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat Gas antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat Gas in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.