BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胰高血糖素样肽 2(Glp-2)是一个 33 个氨基酸的肽。GLP-2 调节胃肠蠕动、胃酸分泌、肠道己糖运输，并增加肠道上皮细胞的屏障功能。它通过刺激隐窝细胞增殖、抑制肠细胞和隐窝的细胞凋亡，明显增强了粘膜上皮的表面积。在小肠和大肠炎症的情况下，GLP-2 能降低死亡率，减少粘膜损伤。它的作用是由 GLP-2 受体转导的，GLP-2 受体是表达在胃、小肠和结肠的肠道内分泌细胞中的 G 蛋白偶联受体。在异源细胞中激活 GLP-2 受体信号能促进对体外凋亡损伤的抵抗。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有大鼠胰高血糖素样肽2(GLP-2)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠胰高血糖素样肽2(GLP-2)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat GLP-2 antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat GLP-2 in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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