BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血管紧张素1-7(ANG 1-7) 分子量=899.02 g/mol，是肾素-血管紧张素系统（RAS）的一种活性七肽。血管紧张素-(1-7)是血管紧张素I与脑微针孵化的主要产物。它已被证明具有抗氧化和抗炎作用。它在自发性高血压的心肌细胞中发挥保护作用，增加内皮细胞和神经元一氧化氮合成酶的表达，从而增加一氧化氮的产生。它在动物模型中引起抗心律失常作用。在血管中诱导释放血管扩张剂，如前列腺素和一氧化氮。此外，它也被证明有助于ACE抑制剂和血管紧张素II受体1型拮抗剂的有益作用。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有大鼠血管紧张素1-7(Ang 1-7)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠血管紧张素1-7(Ang 1-7)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat Ang 1-7 antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat Ang 1-7 in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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