计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
降钙素基因相关肽( Calcitonin gene related peptide.CGRP) ,是人类用分子生物学方法发现的第一个活性多肽,由37个氨基酸组成,分子量约为3800 道尔顿,生物半衰期约为18分钟。CGRP广泛分布于中枢、外周和其他系统中。在中枢神经系统CGRP主要分布于杏仁核、尾核、脊髓脊角和三叉神经束, 垂体、下丘脑、延髓和海马也有一定分布,脊髓中含量最高,大脑皮层其含量则极低。但其受体密度高,均有较多的特异性结合点。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有大鼠降钙素基因相关肽(CGRP)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠降钙素基因相关肽(CGRP)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat CGRP antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat CGRP in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.