BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
25羟基维生素D3(25(OH)D3)又称骨化二醇，钙化醇或25-羟基胆钙化醇，是维生素D25-羟化酶对维生素D3（胆钙化醇）进行羟化而在肝脏中产生的一种维生素D形式。它可由25(OH)D-1α-羟化酶进一步羟化，主要在肾脏，形成钙三醇（1,25-(OH)2D3），这是维生素D的活性激素形式。它在血液中被维生素D结合蛋白强烈结合。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有小鼠25羟基维生素D3(25(OH)D3)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠25羟基维生素D3(25(OH)D3)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse OHD3 antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse OHD3 in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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