BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
C-型钠尿肽(CNP)是一种 22 个氨基酸的多肽，由 NPPC 基因编码。它属于 钠尿肽家族。它是代谢平衡的一个关键调节因子。它与心钠肽 ANP 和 BNP 具有相似的结构和共同的受体，但 CNP 与它们相比，它主要从血管内皮分泌。 它已被证明对动脉和静脉都有扩张血管的作用，并且在培养中对血管平滑肌 细胞也有抗增殖作用。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有小鼠C型钠尿肽(CNP)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠C型钠尿肽(CNP)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse CNP antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse CNP in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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