计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
P物质(SP)是速激肽神经肽家族中的一个十一肽(由11个氨基酸残基链组成的肽)。它是一种神经肽,可作为一种神经递质和神经调节剂。P物质及其密切相关的神经激肽A(NKA)是由前激肽A基因的不同剪接后的多肽前体产生。它的内源性受体是神经激肽1受体(NK1-receptor,NK1R),属于GPCRs的速激肽受体亚家族。负责结合SP及其拮抗剂的氨基酸残基存在于NK-1的胞外环和跨膜区。SP与NK-1R的结合导致通过clathrin依赖性机制内化到酸化的内体中,在那里复合物被分离出来。随后,SP被降解,NK-1R在细胞表面重新表达。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有小鼠P物质(SP)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠P物质(SP)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse SP antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse SP in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.