BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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促甲状腺激素（又称促甲状腺素、促甲状腺激素，或简称TSH）是由垂体前叶的甲状腺细胞产生的一种糖蛋白激素, 可刺激甲状腺产生甲状腺素，然后产生三碘甲状腺原氨酸，刺激体内几乎所有组织的代谢。甲状腺素和三碘甲状腺原氨酸对维持身体的代谢率、心脏和消化功能、肌肉控制、大脑发育和骨骼维护至关重要。当促甲状腺激素与甲状腺细胞上的受体结合时，会使这些细胞产生甲状腺素和三碘甲状腺原氨酸并释放到血液中。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有小鼠促甲状腺素(TSH)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠促甲状腺素(TSH)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse TSH antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse TSH in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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