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促甲状腺素释放激素（TRH）是由下丘脑分泌的一种三肽激素，化学本质为焦谷氨酸-组氨酸-脯氨酸。其主要作用是刺激垂体前叶分泌促甲状腺激素（TSH），进而促进甲状腺合成和分泌甲状腺激素，调节机体的代谢率和多种生理功能。TRH的分泌受多种因素影响，如神经调节、体内激素水平及环境因素等。在甲状腺功能异常的诊断中，TRH也具有重要意义。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有小鼠促甲状腺素释放激素(TRH)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠促甲状腺素释放激素(TRH)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse TRH antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse TRH in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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