计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
环磷酸鸟苷(cGMP)是一种环状核苷酸,来源于三磷酸鸟苷(GTP)。它最可能的作用机制是激活细胞内的蛋白激酶,以应对膜渗透性肽类激素与细胞外表面的结合。鸟苷酸环化酶(GC)催化 cGMP 的合成。这种酶将 GTP 转换为 cGMP。肽类激素,如心房钠尿素激活膜结合的 GC,而可溶性 GC( sGC ) 通常被一氧化氮激活以刺激 cGMP 的合成。sGC 可被 ODQ(1H-[1,2,4]噁二唑并[4,3-a]喹喔啉-1-酮)抑制。cGMP 是离子通道传导性、糖原分解和细胞凋亡的常见调节剂。它还能放松平滑肌组织。在血管中,血管平滑肌的放松导致血管扩张和血流量增加。cGMP是眼睛中光传导的二级信使。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有小鼠环磷酸鸟苷(cGMP)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠环磷酸鸟苷(cGMP)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse cGMP antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse cGMP in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.