BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
神经肽FF(NPFF)是一种酰胺化神经肽，被认为是一种疼痛调节肽，对吗啡引起的镇痛有抗阿片类药物的活性。它是由NPFF基因编码的。它和由两种前体发出的RF酰胺相关肽与G蛋白偶联受体的两种亚型，即NPFF1和NPFF2亚型有良好的亲和力，并参与多种生理功能，如心血管调节、荷尔蒙控制、巨噬细胞激活、体温平衡和疼痛调节。它还控制脂肪组织巨噬细胞的数量和代谢作用，它对脂肪组织的健康是必要的。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有小鼠神经肽FF(NPFF)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠神经肽FF(NPFF)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse NPFF antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse NPFF in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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