BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
神经降压素(NT)是一种13个氨基酸的神经肽，因其具有明显的降压作用且存在于神经组织而得名。同时，它与黄体生成素和催乳素释放的调节有关，并与多巴胺能系统有明显的相互作用。它分布于整个中枢神经系统，在下丘脑、杏仁核和阿肯色核中含量最高。它能诱发各种效应，包括镇痛、低温和增加运动性活动。它还参与了对多巴胺途径的调节。在外周，它存在于小肠的肠道内分泌细胞中，它导致分泌和平滑肌收缩。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有小鼠神经降压素(NT)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠神经降压素(NT)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse NT antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse NT in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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