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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胃泌酸调节素(OXM)也称为催产素调节素,是在结肠中发现的天然存在的 37 个氨基酸的肽激素,由促性粘膜的促性细胞产生。已发现它会抑制食欲,它同时结合 GLP-1 受体和胰高血糖素受体。催产素调节素与肝脏生物钟的夹带有关。催产素调节素已被研究为与糖尿病有关的血糖调节剂。OXM 可以代表约 40% 的肠胰高血糖体,在回肠中观察到的浓度最高。OXM 在肝脏和脂肪组织中的效力不如胰高血糖素,因为它的 C 端八肽。然而,它通过独特的受体在胃粘膜抑制中显示出独特的功效。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有小鼠胃泌酸调节素(OXM)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠胃泌酸调节素(OXM)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse OXM antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse OXM in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.