胆囊收缩素/肠促胰酶肽(CCK)是胃肠系统的一种肽类激素，负责刺激脂肪和蛋白质的消化。胆囊收缩素，正式名称为胰岛素，由十二指肠（小肠的第一段）的肠内分泌细胞合成和分泌。它的存在导致消化酶和胆汁分别从胰腺和胆囊中释放出来，同时也起到抑制饥饿的作用。它是胃泌素/胆囊收缩素系列多肽激素的成员，在结构上与另一种胃肠道激素胃泌素非常相似。CCK和胃泌素有相同的五个C端氨基酸。CCK由不同数量的氨基酸组成，取决于150个氨基酸前体的翻译后修饰，即前囊素。因此，CCK多肽激素以多种形式存在，每种形式以其包含的氨基酸数量来识别，如CCK58、CCK33、CCK22和CCK8。CCK58呈螺旋-转折-螺旋构型。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有小鼠胆囊收缩素/肠促胰酶肽(CCK)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠胆囊收缩素/肠促胰酶肽(CCK)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse CCK antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse CCK in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
危险品化学品经营许可证（带存储）