BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血管活性肠肽(VIP) 也被称为血管活性肠多肽，是一种在肠道中具有血管活性的肽类激素。它是一种由28个氨基酸残基组成的多肽，属于胰高血糖素/分泌素超家族，是第二类G蛋白偶联受体的配体。VIP在脊椎动物的许多组织中产生，包括肠道、胰腺和大脑中下丘脑的上核。它刺激心脏的收缩性，引起血管扩张，增加糖原分解，降低动脉血压，放松气管、胃和胆囊的平滑肌。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有小鼠血管活性肠肽(VIP)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠血管活性肠肽(VIP)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse VIP antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse VIP in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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