计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血管紧张素I又称称为原血管紧张素,由肾素对血管紧张素原的作用形成。肾素裂解血管紧张素原上的亮氨酸(Leu)和缬氨酸(Val)残基之间的肽键,形成十肽(十个氨基酸)(des-Asp)血管紧张素I。肾素在肾交感神经活动、肾内压力下降(收缩压<90mmHg[10])时在肾小球细胞中产生,或向大网膜输送的Na+和Cl-减少。如果大网膜感觉到远端小管中NaCl浓度降低,大网膜细胞释放的肾素会增加。血管紧张素I似乎没有直接的生物活性,仅作为血管紧张素II的前体而存在。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有小鼠血管紧张素Ⅰ(Ang-Ⅰ)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠血管紧张素Ⅰ(Ang-Ⅰ)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse Ang-Ⅰ antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse Ang-Ⅰ in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.