计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
酪酪肽(PYY)也被称为肽酪氨酸(peptide tyrosine),是一种由PYY基因编码的多肽。肽YY是一种短的(36个氨基酸)多肽,由回肠和结肠的细胞在进食时释放。在血液、肠道和外围的其他元素中,PYY的作用是降低食欲;同样,当直接注射到中枢神经系统时,PYY也具有厌食性,即降低食欲。来自水果、蔬菜和全谷物的膳食纤维,食用后可增加肠道食糜进入回肠的速度,以提高PYY3-36,并诱发饱腹感。肽YY可以作为粗鱼蛋白酶解的结果产生,并作为食品摄入。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有小鼠酪酪肽(PYY)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠酪酪肽(PYY)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse PYY antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse PYY in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.