BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
酰基化饥饿素(AG)是由胃肠道，特别是胃的肠内分泌细胞产生的一种激素， 通常被称为 "饥饿激素"，因为它增加了进食的动力。AG是胃黏膜分泌的28氨基酸多肽激素，通过第3位丝氨酸的辛酰基修饰激活，是唯一已知的胃肠道促食欲激素。只有AG能特异性结合生长激素促分泌受体（GHSR-1a），激活下游信号通路。刺激生长激素释放、促进摄食行为，参与能量稳态平衡。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有小鼠酰基化饥饿素(AG)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠酰基化饥饿素(AG)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse AG antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse AG in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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