计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
雄激素(androgen)主要是由睾丸合成和分泌,肾上腺和卵巢也能分泌少量。 天然的雄性激素为睾丸素(睾酮),具有雄激素活性,并有一定的蛋白质同 化作用。雄激素是对附睾结构,功能影响最大的激素。附睾液中雄激素含量 很高,其主要来源于睾网液和血循环。此外一些研究认为附睾上皮也能合成 和分泌雄激素,因为实验证明附睾上皮中有 LH 受体和 5α还原酶等一些雄激 素代谢有关的酶 mRNA 的表达。附睾液中的雄激素主要是睾酮和 DHT,它 们在附睾体部含量最高,与精子的成熟有关。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有小鼠雄激素(Androgen)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠雄激素(Androgen)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse Androgen antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse Androgen in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.