计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
食欲素A又称下丘脑素-1,是一种天然存在的神经肽和奥曲肽的异构体。位 于下丘脑外侧的奥曲肽核是大脑中主要的奥曲肽投射系统。它是一个由33个 氨基酸组成的多肽,包括一个N端焦谷氨酸残基和位于6和12以及7和14位置 的半胱氨酸残基之间的两个分子内二硫桥。奥曲肽是高度兴奋性的神经肽。 它是由下丘脑外侧和后侧的极少数细胞群产生的一种肽。奥曲肽强烈激发各 种脑核(神经元),通过影响其多巴胺、去甲肾上腺素、组胺和乙酰胆碱系 统来影响生物体的清醒状态。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有小鼠食欲素A(OXA)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠食欲素A(OXA)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse OXA antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse OXA in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.