BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Adropin蛋白(AD)是一种多肽激素，由Kumar等人在2008年发现，这种蛋白质由76个氨基酸组成，它最初被描述为一种分泌肽，其中1-33号残基编码一个分泌信号肽序列。该蛋白由Enho基因编码，主要在肝脏和中枢神经系统中表达。它参与碳水化合物-脂质代谢、代谢性疾病、中枢神经系统功能、内皮功能和心血管疾病。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有人Adropin蛋白(AD)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Adropin蛋白(AD)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human AD antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human AD in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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