计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Apelin 12(AP12)是最有效的多肽 C 端片段之一,对孤儿受体 APJ 受体具有 高亲和力。它参与体液平衡的调节和进食的中枢控制。它能阻止 HIV-1 通过 APJ 受体进入体内。Apelin-12 具有神经保护作用。Apelin-12 是 apelin 家族的一员,是人孤儿 G 蛋白偶联受体 (APJ) 的配体。Apelin/APJ 可以减少 HG 诱导的内皮细胞中 ROS 形成和凋亡。Apelin-12 是 Apelin 最有效的短肽物质之一,对心血管损伤具有保护作用,对应于氧化应激。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有人apelin 12(AP12)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人apelin 12(AP12)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human AP12 antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human AP12 in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.