BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Apelin-36 是一种具有很强抗炎活性的内源性活性肽。是 Apelin 前体蛋白的长肽片段，广泛分布在中枢神经系统及其外周组织中，并发挥抗炎和抗氧化应激作用。例如，Apelin-36 抑制脑缺血再灌注（I/R）损伤诱导的 CHOP 和 GRP78 的增加，Apelin-36 还可以通过抑制凋亡信号来调节 ASK1/JNK/Caspase-3 通路，并调节氧化应激、自噬和细胞凋亡以发挥神经保护作用。此外，Apelin-36 通过抑制 ASK1/MAPK 信号通路保护脂多糖（LPS）诱导的急性肺损伤。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有人Apelin 36(AP36)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Apelin 36(AP36)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human AP36 antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human AP36 in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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