BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
促胰液素(secretin)是一种调节全身水分平衡的激素，通过调节胃、胰腺和肝 脏的分泌物来影响十二指肠的环境。它是一种多肽激素，产生于十二指肠的S 细胞，这些细胞位于肠道腺体中。它通过以下方式帮助调节十二指肠的 pH 值：抑制胃顶细胞分泌胃酸；刺激胰腺导管细胞产生碳酸氢盐。它还刺激肝脏产生胆汁；胆汁将十二指肠中的食物脂肪乳化，使胰腺脂肪酶能够作用于它们。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有人促胰液素(secretin)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人促胰液素(secretin)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human secretin antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human secretin in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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