计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
催产素(OT)是一种多肽激素和神经肽,通常由下丘脑产生,并由垂体后叶释 放它在社会联系、生殖、分娩和产后期间发挥着作用。无论是哪种形式,催 产素都能刺激子宫收缩,加速分娩过程。催产素的生产和分泌受正反馈机制 的控制,它的最初释放会刺激进一步催产素的生产和释放。例如,当催产素 在分娩开始时子宫收缩时释放,这将刺激更多催产素的产生和释放,并增加 收缩的强度和频率。这个过程在强度和频率上不断增加,一直持续到触发活 动停止。类似的过程在哺乳期和性活动中也会发生。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有人催产素(OT)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人催产素(OT)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human OT antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human OT in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.