BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
前列腺素F2α(PGF2α)药学上称为卡前列素，是一种天然存在的前列腺素， 在医学上用于诱导分娩和堕胎。它作用于黄体，引起黄体溶解，形成白体并 停止孕酮的产生。PGF2α的作用取决于黄体膜上受体的数量。PGF2α通过与 前列腺素F2α受体结合发挥作用。它与子宫催产素水平的增加有关，有证据 表明PGF2α和催产素形成一个正反馈回路，以促进黄体的退化。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有人前列腺素F2α(PGF2α)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人前列腺素F2α(PGF2α)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human PGF2α antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human PGF2α in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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