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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
松弛肽 3/松弛素 3(RLN-3)是一种一种神经肽。它主要在大脑的神经元内表 达,在那里它被包装成致密的核小泡并沿轴突运输,在释放到目标神经元之 前可以在突触前的小泡中检测到,这是神经递质的特征。啮齿动物大脑中数 量最多的松弛素-3 阳性神经元位于被称为 "无核 "的桥脑干区域,而较小的 群体存在于桥脑棘突、弯管周围灰和黑质背侧的一个区域。从这些中心出发, 松弛素-3 支配广泛的脑区,这些脑区也有丰富的 RXFP3 mRNA/结合位点, 包括扩展的边缘系统和海马区通路。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有人松弛肽3/松弛素3(RLN-3)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人松弛肽3/松弛素3(RLN-3)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human RLN-3 antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human RLN-3 in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.