计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Galanin是一种由GAL基因编码的抑制性、超极化的神经肽,能抑制神经递质的释放,在人类和其他哺乳动物的大脑、脊髓和肠道中广泛表达。Galanin的信号是通过三个G蛋白偶联受体发生的。它与许多生物学上的不同功能有关,包括:痛觉、觉醒和睡眠调节、认知、进食、情绪调节、血压调节,它还在发育中发挥作用,并作为一种营养因子发挥作用。Galanin还具有神经保护活性,因为当周围神经系统的轴切以及大脑发生癫痫活动时,其生物合成增加2-10倍。它还可能促进神经的形成,通常与经典的神经递质如乙酰胆碱、5-羟色胺和去甲肾上腺素共同定位,也与其他神经调节剂如神经肽Y、P物质和血管活性肠肽共同定位。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有人甘丙肽(GAL)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人甘丙肽(GAL)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human GAL antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human GAL in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.