计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
神经肽是由小链子的氨基酸组成的化学信使,由神经元合成和释放。它通常与 G 蛋白偶联受体(GPCRs)结合,调节神经活动和其他组织,如肠道、肌肉和心脏。有超过 100 种已知的神经肽,代表了神经系统中最大和最多样化的一类信号分子。神经肽是由大型前体蛋白合成的,这些前体蛋白经过裂解和翻译后处理,然后被包装成密集的核心囊泡。它经常与其他神经肽和神经递质在单个神经元中共同释放,产生多种效应。一旦释放,它可以广泛扩散以影响广泛的目标。神经肽是由被称为前肽的大型无活性前体蛋白合成的。前肽包含一系列不同的肽的序列,并且常常包含相同肽的重复拷贝,这取决于生物体。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有人神经肽(NPB)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人神经肽(NPB)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human NPB antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human NPB in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.