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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
缓激肽是一种促进炎症的肽。它通过释放前列环素、一氧化氮和内皮衍生的超极化因子使动脉血管扩张(扩大),并通过前列腺素 F2 使静脉收缩,从而导致毛细血管床渗漏,这是由于毛细血管中的压力增加。缓激肽是一种具有生理和药理活性的激肽,属于激肽类蛋白质,由九个氨基酸组成。一类称为血管紧张素转换酶抑制剂(ACE 抑制剂)的药物通过抑制缓激肽的降解来提高其水平,从而增加其降压效果。ACE 抑制剂被 FDA 批准用于治疗高血压和心衰。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有人缓激肽(BK)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人缓激肽(BK)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human BK antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human BK in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.