计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胃泌素是一种多肽激素,可刺激胃顶细胞分泌胃酸(HCl)并帮助胃部运动。 它由胃、十二指肠和胰腺的幽门前的 G 细胞释放。胃泌素与胆囊收缩素 B 受 体结合,刺激肠铬素样细胞释放组胺,并诱导 K+/H+ ATP 酶泵插入顶层细 胞的顶端膜(这反过来又增加 H+释放到胃腔)。它的释放受到胃腔内肽类 的刺激。在佐林格-艾利森综合征中,胃泌素的产生水平过高,通常是由胃泌 素瘤产生的胃泌素肿瘤,大多数是十二指肠或胰腺的良性肿瘤。为了调查高 胃泌素血症的情况,可以进行 "五肽胃泌素试验"。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有人胃泌素(Gas)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人胃泌素(Gas)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human Gas antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human Gas in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.