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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胃泌素是一种酸性胃肠激素,对调节消化道功能和维持其结构完整有重要作用。人体中 95%以上有生物活性的胃泌素是α-酰胺化胃泌素,可分为胃泌素17gastrin 17,G17)和胃泌素 34(gastrin 34,G34)两种异构体,其中80%~90%是 G17。研究表明,G-17 仅由胃窦部 G 细胞分泌,因此它被认为是反应胃黏膜菱缩性胃炎的程度和范围的重要指标。血清胃泌素 17 与胆囊收缩素受体(CCKR))结合后通过一系列信号转导而发生生物学效应,主要参与刺激胃酸分泌及营养胃肠道粘膜。在慢性萎缩性胃炎中,血清 G17 与胃黏膜萎缩部位及萎缩程度具有相关性。胃窦彭缩时,G 细胞数量较少,血清G17 水平下降;胃体萎缩时泌酸细胞数量减少,胃内处于低酸状态,由胃泌素胃酸轴负反馈导致血清 G17 升高。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有人胃泌素17(GAS17)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人胃泌素17(GAS17)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human GAS17 antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human GAS17 in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.