计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胸腺素β4(Tβ4)是一种蛋白质,由TMSB4X基因编码。它由43个氨基酸组成,分子量为4921 g/mol。它属于一种肌动蛋白封闭蛋白,在调节肌动蛋白聚合方面起作用。它还参与了细胞增殖、迁移和分化。该基因能逃避X的失活,并在Y染色体上有一个同源物(TMSB4Y)。胸腺肽-β4 是许多组织中的主要细胞成分。其细胞内浓度可高达 0.5 mM。继胸腺肽 α1 之后,β4 是胸腺肽组分 5 中第二个被完全测序和合成的生物活性肽。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有人胸腺素β4(Tβ4)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人胸腺素β4(Tβ4)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human Tβ4 antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human Tβ4 in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.