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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
速激肽 1(TAC1)是一种前体蛋白,由 TAC1 基因编码。该蛋白质具有四种 亚型-α,β,γ-和δ-PPT-它们可以进行翻译后修饰以产生神经激肽 A(以前 称为物质 K)和物质 P。α和δ-PPT 只能被修饰成 P 物质,而β和γ-PPT 可以 产生 P 物质和神经激肽 A。神经激肽 A 还可以进一步修饰以产生神经肽 K(也 称为神经激肽 K)和神经肽γ。这些激素被认为是神经递质,与神经受体和平 滑肌细胞相互作用。众所周知,它们可诱导行为反应,并起血管扩张剂和促 分泌剂的作用。外显子 4 和/或 6 的替代剪接产生四个意义不明的已知产物。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有人速激肽1(TAC1)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人速激肽1(TAC1)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human TAC1 antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human TAC1 in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.