BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
阿黑皮素原也称原阿片皮质素（POMC），是一个有241个氨基酸残基的前体多肽。POMC在垂体前叶的皮质激素中由267个氨基酸长的多肽前体原-原黑皮素（pre-POMC）合成，在翻译过程中去除26个氨基酸长的信号肽序列。POMC被切割（裂解）后产生多种肽类激素。这些多肽中的每一种都被包装在大的密核囊泡中，在适当的刺激下通过外渗从细胞中释放。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有人阿黑皮素原(POMC)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人阿黑皮素原(POMC)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human POMC antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human POMC in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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