BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
内皮素2(ET-2)是由ET-2基因编码的一种蛋白质。它属于三个内皮素多肽异构体（ET-1、ET-2、ET-3）的家族，可使血管收缩。它由不同的染色体上的基因对其异构体进行编码，主要产生于肾脏、胎盘、子宫、心脏、中枢神经系统和肠道的血管内皮细胞。它通过与两个不同的G蛋白偶联受体（GCPRs）结合而发挥作用，即内皮素A受体（EDNRA）和内皮素B受体（EDNRB）。 本试剂盒采用竞争法酶联免疫吸附试验（ELISA）。往预先包被有大鼠内皮素2(ET-2)抗原的微孔中，依次加入样本、标准品、Biotin标记的抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠内皮素2(ET-2)呈负相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat ET-2 antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat ET-2 in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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