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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
多粘菌素 B 是一种用于治疗脑膜炎、肺炎、败血症和尿路感染的抗生素。虽然它对许多革兰氏阴性菌感染有用,但对革兰氏阳性菌感染没有用。它可以通过静脉、肌肉或脑脊液注射或吸入给药。通常仅在没有其他选项时才使用可注射形式。它也可以作为杆菌肽/多粘菌素 B 和新霉素/多粘菌素 B/杆菌肽的组合用于皮肤。多粘菌素 B 由多粘菌素 B1、B1-I、B2、B3 和 B6 组成。多粘菌素 B1 和 B2 被认为是主要成分。这些相关组分在结构上是相同的,除了每个馏分上的可变脂肪酸基团。体外研究的结果表明,在比较馏分时,C 数据存在边际差异。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有多粘菌素B(Polymyxin B)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的多粘菌素B(Polymyxin B)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Polymyxin B antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Polymyxin B in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.