计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
内毒素(ET)也称为脂多糖 (LPS),是革兰氏阴性菌(如大肠杆菌和沙门氏菌)细胞包膜最外层膜成分的统称,具有共同的结构结构。脂多糖是由三部分组成的大分子:称为 O 抗原的外核多糖、内核低聚糖和脂质 A(毒性主要来源),均共价连接。在目前的术语中,术语内毒素通常与 LPS 同义,尽管有一些内毒素(最初意义上的细菌细胞内部的毒素,当细胞分解时释放)与 LPS 无关,例如苏云金芽孢杆菌产生的所谓 δ 内毒素蛋白。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有小鼠内毒素(ET)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠内毒素(ET)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse ET antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse ET in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.