计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
雄烯二酮或 4-雄烯二酮(简称 A4 或Δ4-二酮),又称雄-4-烯-3,17-二酮, 是一种内源性弱雄激素类固醇激素,是雌酮和睾酮从脱氢表雄酮(DHEA) 生物合成的中间产物。它与雄烯二醇(androst-5-ene-3β,17β-diol)密切 相关。它是睾酮和其他雄性激素的前体,也是雌性激素如雌酮在体内的前体。 除了作为一种内源性原激素发挥作用外,雄烯二酮本身也具有弱的雄性激素 活性。已发现雄烯二酮具有一定的雌激素活性,与其他 DHEA 代谢物相似。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有雄烯二酮(ASD)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的雄烯二酮(ASD)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Androstenedione (ASD) antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Androstenedione (ASD) in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.