BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Tiragolumab是一种新型免疫检查点抑制剂，该单克隆抗体靶向TIGIT且具完整的Fc区域。 TIGIT是一种新型的抑制性免疫检查点，主要在T细胞和自然杀伤（NK）细胞表面表达，它可以抑制人体对癌症的免疫反应。 TIGIT通路与PD-L1/PD-1通路互补，因此tiragolumab和atezolizumab双重阻断可能有助于克服免疫抑制并恢复免疫应答。 本试剂盒采用间接法酶联免疫吸附试验（ELISA）。往预先包被有人TIGIT抗原的微孔中，依次加入样本、标准品、HRP标记的二抗，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的替瑞利尤单抗(Tiragolumab)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human TIGIT antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Tiragolumab in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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