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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
吉伏组单抗是一种实验性单克隆抗体,具有变构调节特性。它能够与促炎性细胞因子白细胞介素 - 1β(IL-1β)结合,负向调节产生炎症的细胞信号传导事件。IL-1β 与心血管疾病、肺癌和自身炎症性疾病等多种疾病的发生发展相关。吉伏组单抗曾被用于痤疮、骨关节炎、白塞氏葡萄膜炎、坏疽性脓皮病等疾病治疗的试验研究。 本试剂盒采用间接法酶联免疫吸附试验(ELISA)。往预先包被有人IL-1β抗原的微孔中,依次加入样本、标准品、HRP标记的二抗,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的吉伏组单抗(Gevokizumab)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human IL-1β antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Gevokizumab in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.