BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Elotuzumab 是一种免疫刺激性抗体，靶向信号淋巴细胞活化分子家族成员 7，也称为 SLAMF7。SLAMF7 是一种细胞表面糖蛋白，存在于骨髓瘤细胞、自然杀伤细胞、浆细胞和造血谱系免疫细胞亚群上。 Elotuzumab 的工作原理是通过 SLAMF7 通路激活自然杀伤细胞。与此同时，骨髓瘤细胞的 SLAMF7 被靶向和标记，通过抗体依赖性细胞毒性进行自然杀伤细胞介导的破坏。 本试剂盒采用间接法酶联免疫吸附试验（ELISA）。往预先包被有人SLAMF7抗原的微孔中，依次加入样本、标准品、HRP标记的二抗，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的埃罗妥珠单抗(Elotuzumab)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样本浓度。
This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human SLAMF7 antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Elotuzumab in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.
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